Custom-designed zinc finger nucleases: What is next?
نویسندگان
چکیده
منابع مشابه
Zinc finger nucleases: custom-designed molecular scissors for genome engineering of plant and mammalian cells
Custom-designed zinc finger nucleases (ZFNs), proteins designed to cut at specific DNA sequences, are becoming powerful tools in gene targeting--the process of replacing a gene within a genome by homologous recombination (HR). ZFNs that combine the non-specific cleavage domain (N) of FokI endonuclease with zinc finger proteins (ZFPs) offer a general way to deliver a site-specific double-strand ...
متن کاملSite-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases.
Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation for an endogenous gene in Arabidopsis using zinc finger nucleases (ZFNs). Engineered ZFNs for a stress-response regulator, the ABA-INSENSITIVE4 (ABI4) gene, cleaved their recognition sequences specifically in vitro, and Z...
متن کاملCustom zinc-finger nucleases for use in human cells.
Genome engineering through homologous recombination (HR) is a powerful instrument for studying biological pathways or creating treatment options for genetic disorders. In mammalian cells HR is rare but the creation of targeted DNA double-strand breaks stimulates HR significantly. Here, we present a method to generate, evaluate, and optimize rationally designed endonucleases that promote HR. The...
متن کاملEnhancing gene targeting with designed zinc finger nucleases.
Gene targeting—the process of gene replacement by homologous recombination—is a very useful but typically inefficient technique (1). A general method for improving the efficiency of gene targeting would be valuable in many circumstances, as would the extension of this genetic tool to a broader range of organisms. Model experiments have demonstrated that a double-strand break (DSB) in the chromo...
متن کاملCell Biology Symposium: Zinc finger nucleases to create custom-designed modifications in the swine (Sus scrofa) genome.
Engineered zinc finger nucleases (ZFN) are rapidly gaining popularity as a means to enhance the rate and specificity of DNA modifications in plant and animal cells. Repair-mediated gene modification by ZFN is driven by introducing DNA double-strand breaks via a nonspecific nuclease domain linked to a sequence-specific zinc finger nucleotide recognition domain. This review examines the use of ZF...
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ژورنال
عنوان ژورنال: Cellular and Molecular Life Sciences
سال: 2007
ISSN: 1420-682X,1420-9071
DOI: 10.1007/s00018-007-7206-8